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cd326 microbeads  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd326 microbeads
    Cd326 Microbeads, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 96/100, based on 193 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd326+microbeads/CD326+(EpCAM)+MicroBeads%2C+human/us12668765-85-5-7
    Average 96 stars, based on 193 article reviews
    cd326 microbeads - by Bioz Stars, 2026-10
    96/100 stars

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    Suspension:

    Article Title: Microfluidic system simulating lung tissue
    Article Snippet: Thereafter, in order to increase the purity of the isolated cells, 100 ul of the FcR blocking reagent (Miltenyi Biotec, #130-059-901) was added to the lung epithelial cell suspension, and the FcR blocking reagent was not added to the lung fibroblast suspension. .. Thereafter, 100 ul of the CD326 microbeads (Miltenyi Biotec, #130-061-101) and 20 ul of the anti-fibroblast microbeads (Miltenyi Biotec, #130-050-601) were added to the lung epithelial cell suspension and the lung fibroblast suspension, respectively, and each was subjected to a vortex to be well mixed. ..

    Selection:

    Article Title: Phenotypic profiling of neutrophils in acute Clostridioides difficile infection identifies a TNF-induced activation signature associated with epithelial damage
    Article Snippet: TNF levels in cecal homogenates were quantified using an ELISA kit (BioLegend #430901) and normalized to total protein measured using a BCA Protein Assay Kit (Thermo Fisher #23225). .. Neutrophils were enriched using negative selection kits (as described above), and epithelial cells were further depleted using CD326 MicroBeads (Miltenyi #130-105-958). .. Neutrophil sorting buffer was supplemented with 1 U/μl RNase inhibitor (Millipore Sigma RNAINH-RO).

    Article Title: Phenotypic profiling of neutrophils in acute Clostridioides difficile infection identifies a TNF-induced activation signature associated with epithelial damage.
    Article Snippet: TNF levels in cecal homogenates were quantified using an ELISA kit (BioLegend #430901) and normalized to total protein measured using a BCA Protein Assay Kit (Thermo Fisher #23225). .. Neutrophils were enriched using negative selection kits (as described above), and epithelial cells were further depleted using CD326 MicroBeads (Miltenyi #130-105-958). .. Neutrophil sorting buffer was supplemented with 1 U/μl RNase inhibitor (Millipore Sigma RNAINH-RO).

    Article Title: Late-stage dedifferentiation and epigenetic memory of cancer stem cells in hepatocellular carcinoma.
    Article Snippet: Keywords Hepatocellular carcinoma, Cancer stem cells, Clonal evolution, Single-cell somatic mutation, Bipotent progenitor, Dedifferentiation, DNA methylation centrifuged at 450g for 10 min. After ACK red blood cell lysis and re-filtration, Dead Cell Removal Kit (Miltenyi, 130–090-101) was applied when cell viability fell below 80%. .. EpCAM magnetic bead enrichment and 10x genomics sequencing A total of 2 × 107 cells were resuspended in 300 μl MACS buffer (PBS + 0.5% BSA + 2 mM EDTA), supplemented with 100 μl CD326 microbeads (Miltenyi, 130–045-801) and 100 μl Fc block, and incubated at 4 °C for 30 min. After washing and filtration through 40 μm cell strainers, positive selection was performed on MS columns using a magnetic separator. ..

    Isolation:

    Article Title: Targeting CREB remodels the immune microenvironment to enhance immunotherapy responses in pancreatic cancer
    Article Snippet: .. For quantification of LIF in orthotopic KPC PDAC tumors, CD326 + (EpCAM) cells were isolated by using CD326 + microbeads (Miltenyi 130-105-958), a MIDIMACS separator and LS columns (#130-042-401). ..

    Article Title: Human embryonic stem cell-derived Sertoli cells as an immune modulator of cell transplantation therapy in a diabetic mice model.
    Article Snippet: .. To evaluate whether hESC-derived IM (hESC-IM) can expand, hESC-IM was isolated on the fourth day after IM induction and subcultured. hESC-IM was first negatively sorted with CD326 microbeads (Miltenyi Biotech), and then sorted with CD26 microbeads (Miltenyi Biotech) according to the manufacturer’s instructions [26]. .. CD326(-)CD56(+) cells were seeded onto Geltrex (Gibco)-coated 6-well plates in A-RPMI 1640 supplemented with 1% GultaMAX, 1% P/S, 100 ng/mL hrbFGF (Peprotech), and 10% FBS (Gibco), cultured, and then passaged using trypsin-EDTA when they reached ≥ 95% confluence.

    Article Title: Sustained Yap/Taz activation promotes aberrant alveolar epithelial cell differentiation and drives persistent fibrotic remodeling
    Article Snippet: Cell suspensions were generated in C tubes (Miltenyi Biotec 130-093-237) using a gentleMACS dissociator then passed through 100 μm and 70μm filters (MTC Bio C4100 and C4070) to achieve single-cell suspensions. .. Epithelial cells were isolated by incubating cells with CD326 + microbeads (Miltenyi Biotec 130-061-101) and passing through LS columns (Miltenyi Biotec 130-042-401) positioned on a magnetic stand. .. CD326 + cells were plated in Matrigel droplets (Corning 356231) with serum-free feeder-free media (SFFFM) and expanded.

    Incubation:

    Article Title: Evaluation and modification of tumor cell isolation techniques from malignant effusions for rapid drug sensitivity testing
    Article Snippet: Isolation of EpCAM‐positive cells was performed using MACS cell separation system (Miltenyi Biotec, Bergisch Gladbach, Germany) according to the manufacturer's instructions. .. Briefly, the cells were blocked using an anti‐FcR reagent (Miltenyi Biotec, 130‐059‐901) and then incubated at 4 °C with 100 μL of CD326 MicroBeads (Miltenyi Biotec, 130‐061‐101) for 30 min. .. Following an Isolation buffer wash of unbound beads, the magnetically labeled cells were then separated by passing them through MS columns (Miltenyi Biotec, 130‐042‐201) attached to a magnet (Miltenyi Biotec, MiniMACSTM Separator 130‐042‐102, MACS® MultiStand 130‐042‐303).

    Article Title: Late-stage dedifferentiation and epigenetic memory of cancer stem cells in hepatocellular carcinoma.
    Article Snippet: Keywords Hepatocellular carcinoma, Cancer stem cells, Clonal evolution, Single-cell somatic mutation, Bipotent progenitor, Dedifferentiation, DNA methylation centrifuged at 450g for 10 min. After ACK red blood cell lysis and re-filtration, Dead Cell Removal Kit (Miltenyi, 130–090-101) was applied when cell viability fell below 80%. .. EpCAM magnetic bead enrichment and 10x genomics sequencing A total of 2 × 107 cells were resuspended in 300 μl MACS buffer (PBS + 0.5% BSA + 2 mM EDTA), supplemented with 100 μl CD326 microbeads (Miltenyi, 130–045-801) and 100 μl Fc block, and incubated at 4 °C for 30 min. After washing and filtration through 40 μm cell strainers, positive selection was performed on MS columns using a magnetic separator. ..

    Sequencing:

    Article Title: Late-stage dedifferentiation and epigenetic memory of cancer stem cells in hepatocellular carcinoma.
    Article Snippet: Keywords Hepatocellular carcinoma, Cancer stem cells, Clonal evolution, Single-cell somatic mutation, Bipotent progenitor, Dedifferentiation, DNA methylation centrifuged at 450g for 10 min. After ACK red blood cell lysis and re-filtration, Dead Cell Removal Kit (Miltenyi, 130–090-101) was applied when cell viability fell below 80%. .. EpCAM magnetic bead enrichment and 10x genomics sequencing A total of 2 × 107 cells were resuspended in 300 μl MACS buffer (PBS + 0.5% BSA + 2 mM EDTA), supplemented with 100 μl CD326 microbeads (Miltenyi, 130–045-801) and 100 μl Fc block, and incubated at 4 °C for 30 min. After washing and filtration through 40 μm cell strainers, positive selection was performed on MS columns using a magnetic separator. ..

    Magnetic Cell Separation:

    Article Title: Late-stage dedifferentiation and epigenetic memory of cancer stem cells in hepatocellular carcinoma.
    Article Snippet: Keywords Hepatocellular carcinoma, Cancer stem cells, Clonal evolution, Single-cell somatic mutation, Bipotent progenitor, Dedifferentiation, DNA methylation centrifuged at 450g for 10 min. After ACK red blood cell lysis and re-filtration, Dead Cell Removal Kit (Miltenyi, 130–090-101) was applied when cell viability fell below 80%. .. EpCAM magnetic bead enrichment and 10x genomics sequencing A total of 2 × 107 cells were resuspended in 300 μl MACS buffer (PBS + 0.5% BSA + 2 mM EDTA), supplemented with 100 μl CD326 microbeads (Miltenyi, 130–045-801) and 100 μl Fc block, and incubated at 4 °C for 30 min. After washing and filtration through 40 μm cell strainers, positive selection was performed on MS columns using a magnetic separator. ..

    Blocking Assay:

    Article Title: Late-stage dedifferentiation and epigenetic memory of cancer stem cells in hepatocellular carcinoma.
    Article Snippet: Keywords Hepatocellular carcinoma, Cancer stem cells, Clonal evolution, Single-cell somatic mutation, Bipotent progenitor, Dedifferentiation, DNA methylation centrifuged at 450g for 10 min. After ACK red blood cell lysis and re-filtration, Dead Cell Removal Kit (Miltenyi, 130–090-101) was applied when cell viability fell below 80%. .. EpCAM magnetic bead enrichment and 10x genomics sequencing A total of 2 × 107 cells were resuspended in 300 μl MACS buffer (PBS + 0.5% BSA + 2 mM EDTA), supplemented with 100 μl CD326 microbeads (Miltenyi, 130–045-801) and 100 μl Fc block, and incubated at 4 °C for 30 min. After washing and filtration through 40 μm cell strainers, positive selection was performed on MS columns using a magnetic separator. ..

    Filtration:

    Article Title: Late-stage dedifferentiation and epigenetic memory of cancer stem cells in hepatocellular carcinoma.
    Article Snippet: Keywords Hepatocellular carcinoma, Cancer stem cells, Clonal evolution, Single-cell somatic mutation, Bipotent progenitor, Dedifferentiation, DNA methylation centrifuged at 450g for 10 min. After ACK red blood cell lysis and re-filtration, Dead Cell Removal Kit (Miltenyi, 130–090-101) was applied when cell viability fell below 80%. .. EpCAM magnetic bead enrichment and 10x genomics sequencing A total of 2 × 107 cells were resuspended in 300 μl MACS buffer (PBS + 0.5% BSA + 2 mM EDTA), supplemented with 100 μl CD326 microbeads (Miltenyi, 130–045-801) and 100 μl Fc block, and incubated at 4 °C for 30 min. After washing and filtration through 40 μm cell strainers, positive selection was performed on MS columns using a magnetic separator. ..



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